陆川猪ANKRD1基因克隆及其表达差异分析

[目的]克隆陆川猪心肌锚蛋白重复域1基因(ANKRD1),并进行生物信息学及组织表达谱分析,为研究ANKRD1在陆川猪机体内的功能作用提供参考依据.[方法]根据NCBI已公布的野猪ANKRD1基因序列(NM_213922.1)设计特异性引物,采用TRIzol法提取陆川猪心脏、肝脏、脾脏、肺脏、肾脏、背最长肌和皮下脂肪的总RNA,反转录合成cDNA,并以此为模板进行ANKRD1基因克隆,通过MegAlign、Protaram、Protscale、MHMM Server和Sig-nalP等在线分析软件进行生物信息学分析,最后以实时荧光定量PCR检测ANKRD1基因在陆川猪各组织中的表达情况.[结果]陆川猪ANKRD1基因蛋白编码区(CDS)序列全长960 bp,编码319个氨基酸残基,与NCBI已公布野猪ANKRD1基因(NM_213922.1)的CDS序列存在4处碱基突变,但均为同义突变,二者的ANKRD1氨基酸序列同源性为99.6%.陆川猪ANKRD1基因编码蛋白分子量为36125.70 Da,理论等电点(pI)为7.09,属于稳定蛋白,其二级结构中α-螺旋占46.39%、无规则卷曲占39.81%、β-转角占9.09%、延伸链占4.70%;陆川猪ANKRD1蛋白不存在跨膜结构,也无信号肽,有多个磷酸化位点.陆川猪ANKRD1基因在其心脏、肝脏、脾脏、肺脏、肾脏、背最长肌和皮下脂肪等7个组织中均有表达,其中以心脏中的相对表达量最高,显著高于在其他组织中的相对表达量(P<0.05,下同),在脾脏中的相对表达量最低,显著低于在心脏、肝脏、肺脏和背最长肌中的相对表达量.[结论]ANKRD1基因在陆川猪的心脏、肝脏、脾脏、肺脏、肾脏、背最长肌和皮下脂肪等组织中均有表达,且存在明显差异,故推测ANKRD1基因在不同组织中发挥不同作用.     

泡沫比例混合器在液下喷射灭火系统中的应用

提出了环泵泡沫比例混合器和泡沫压力比例混合器在相应灭火系统中的应用、安装和操作中应注意的问题，对两种泡沫比例混合器组成的系统进行了对比，认为环泵比例混合泡沫灭火系统采用正压进水，可以克服负压进水方式存在的缺陷，比较适合油库火灾的扑救；压力比例混合泡沫灭火系统灵活性、匹配性较差，适用于泡沫混合液流量相对不变的火灾扑救。     

甘蓝与萝卜属间杂交及其胚挽救技术优化

以甘蓝显性雄性不育系C08-11为母本，以萝卜自交系Q07-12为父本进行属间杂交，对杂交子房和胚珠进行离体培养，筛选适合甘蓝与萝卜属间杂种胚挽救的适宜取材时间、培养基及外源添加物。结果表明，胚珠培养优于子房培养；所有处理子房培养仅获得3株杂交苗，取材时间以授粉后第9天为宜，B5培养基中添加合适的生长调节剂、水解酪蛋白和活性炭为子房培养所必需；胚珠培养各处理共获得29株杂交苗，适宜取材时间为蕾期授粉后第14天、花期授粉后第16天，对生长调节剂的需求取决于不同取材时期及培养基。     

集雨限灌对旱作马铃薯产量及水分利用效率的影响

在大田条件下研究了集雨有限补偿灌溉对旱区马铃薯产量及水分利用效率的影响。结果表明,补灌能显著提高旱作马铃薯的产量、水分利用效率和经济系数。与对照相比,补灌处理产量增幅在3.99%~21.21%之间,苗期补灌产量高于薯块膨大期补灌,苗期补灌45 mm具有超补偿效应,补灌90 mm具有高补偿效应。低定额补灌有利于水分利用效率和灌水利用效率的提高,且苗期补灌高于薯块膨大期补灌。补灌明显降低了薯块小薯率而提高了大薯率和中薯率,另外单株薯重也提高了,单株薯重与大薯率对马铃薯的产量贡献最大。综合分析认为苗期补灌45 mm和90 mm是最优补灌方案和备选方案。     

促进合作托管造林稳定有序发展的几点建议

以<中共中央国务院关于加快林业发展的决定>颁布为标志,我国林业进入了一个新的发展阶段,合作托管造林迅速发展,但也出现了一些问题,行业发展举步维艰.针对这些问题,应加强企业管理、建立行业自治机构、强化政府监督、建立咨询机构和普及公众知识,以促进合作托管造林这个新兴行业稳定、有序的发展.     

Effect of Collection Rhythms and Season on Rabbit Semen Production

The effects of collection regimen and time of year on rabbit semen production were determined in this study. A total of 14 crossbreed Hyla bucks were used in winter and summer. In each season, rabbits were assigned to two groups. In group 1, (n = 7) rabbits were subjected to an extensive collection regimen (two ejaculates per male, once daily/week) and in group 2, (n = 7) a semi‐intensive semen collection regimen was performed (two ejaculates per male, twice weekly). The traits recorded for each sample were libido, volume, pH, motility, sperm concentration, percentage of alive spermatozoa and sperm abnormalities. The results obtained in this study indicate that when increasing collection frequency, the rate of useful collections decreased (from 0.81 ± 0.017 to 0.69 ± 0.016; p < 0.01). The rate of useful collection also decreased in the transition from winter to summer (from 0.79 ± 0.018 to 0.70 ± 0.017; p < 0.01). Among the ejaculate characteristics studied, only volume/ejaculate (from 0.64 ± 0.015 to 0.53 ± 0.017; p < 0.01) and spermatozoa/ml (from 406 ± 15 to 359 ± 13 million; p < 0.01) appeared negatively affected by collection. In winter fewer volume/ejaculates were produced (0.55 ± 0.015 vs 0.60 ± 0.016 ml; p < 0.01) and fewer spermatozoa/ml (360 ± 14 vs 394 ± 16 million; p < 0.01) than in summer. The doses produced per ejaculate decreased as collection frequency increased, but the number of doses produced per week was higher in the semi‐intensive than the extensive rhythm (26.5 ± 2.1 vs 20.9 ± 1.5; p < 0.01). The results suggest that a semi‐intensive rhythm may be viewed favourably.     

Different biological behaviour of Waldenström macroglobulinemia in two dogs

Waldenström Macroglobulinemia is a low‐grade immunosecretory disorder associated with lymphoid tumours, which is rarely reported in veterinary medicine. In this study, we describe two clinical cases of this rare syndrome in dogs, each characterized by a different onset and clinical course. In one case, a hyperacute onset and aggressive behaviour of the neoplasm was observed. Absolute serum viscosity (SV) was retrospectively evaluated in order to explain clinical findings. Rotational viscosimetry showed good precision in measuring SV. Both dogs had SV values higher than a control groups of healthy dogs although only one subject developed hyperviscosity symptoms and complications. At high paraprotein concentrations, a slight reduction of the M‐component was associated with a marked decrease in SV. Thus, this work suggests that SV assessment is a relevant tool for managing monoclonal gammopathies, whose usefulness should be further confirmed in larger cohorts of dogs.     

Production and characterization of a monoclonal antibody specific to the M serotype of plum pox potyvirus

A monoclonal antibody to an Albanian isolate of plum pox potyvirus (PPV) was obtained (MAbAL), that specifically recognized strain M of this virus. The specificity of MAbAL, assessed by comparative ELISA on 130 PPV isolates of different geographical origin, 22 of which were also tested by comparative IC-PCR, gave consistent and highly reproducible results. MAbAL seems to be elicited by a stable surface determinant that makes it particularly suitable for successful use under a wide range of conditions. MAbAL is an useful addition to the panel of PPV-specific MAbs available to date.     

Use of computer tomography for imaging of Crassicauda grampicola in a Risso's dolphin (Grampus griseus).

A mature male Risso's dolphin (Grampus griseus) stranded along the coasts of Friuli Venezia Giulia, northeast Italy, in May 2001. Parasitic infection with Crassicauda grampicola is often found in the tympanic bullae and pterygoid sinuses in many of the Risso's dolphins examined from the same area. For this reason, it was decided to perform computed tomography of the head to assess this imaging technique for the diagnosis of crassicaudosis in dolphins. A full postmortem examination confirmed the pathologic findings of the computed tomography scan. This technique can be considered a useful adjunct in the diagnosis of cranial crassicaudosis in live dolphins.